Real Time PCR Master Mixes
New England Biolabs, Inc. WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)- 500 rxn
The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This mix contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is compatible with multiple detection methods including turbidity detection real-time fluorescence detection and end-point visualization.
New England Biolabs, Inc. LunaScript RT Master Mix Kit (Primer-free) - 25 rxn
LunaScript RT Master Mix Kit (Primer-free) is an optimized master mix containing all the necessary components for first strand cDNA synthesis except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers enabling maximum cDNA synthesis flexibility. LunaScript RT Master Mix (Primer-free) features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. In addition, the presence of a blue tracking dye provides a visual indicator for the RT step as well as downstream applications.
GenDEPOT amfiSure ONE PCR Master Mix(2X), P7000, 5 x 1 mL
amfiSure ONE PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure PCR Master Mix provides superior yields in both routine and challenging PCR application such as direct PCR using crude extracted DNA, GC rich region PCR, colony PCR, multiplex PCR and etc.
Biochain Institute Inc QCell-Pro One-Step qRT-PCR SuperMix Kit, 400 rxn/PK
BioChain's QCell-Eva One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with intercalator format. QCell-Pro One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with probe based format.
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
Biotium Forget-Me-Not™ Universal Probe Master Mix (500 reactions)
2X Forget-Me-Not™ Universal qPCR Probe Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping. This kit is suitable for all fluorescent probe-based technologies, including hydrolysis probes (such as TaqMan and dual-labeled BHQ probes) and displacement probes (like Molecular Beacons). Forget-Me-Not™ Universal Probe Master Mix shows excellent concordance of results in singleplex and multiplex reactions, has broad instrument compatibility, and can be used in both standard and fast protocols. This kit does not contain ROX reference dye. This product has sufficient reagent for 500 PCR reactions.
Biochain Institute Inc QCell-Eva One-Step qRT-PCR SuperMix Kit, 400 rxn/PK
BioChain's QCell-Eva One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with intercalator format. QCell-Pro One-Step qRT-PCR SuperMix Kit provides a one-step, simple, robust, inexpensive assay for detection and quantitative analysis of gene expression directly from cells or RNA with probe based format.
Roche Diagnostics PRIMER PRE-MIX+QPCR MASTERMIX
PRIMER PRE-MIX+QPCR MASTERMIX *This item is non returnable.
Biochain Institute Inc Power Eva QPCR SuperMix Kit, 200 rxn/PK
BioChain's QPCR SuperMix is a 2x concentration of premix reagent including Hotstart DNA polymerase and specially formulated real time buffer designed for real-time PCR with intercalator format. Eva and SYBR Green QPCR Supermix is in double-stranded cDNA detection format, while Pro QPCR Supermix is in the format of fluorescent probe-based detection assays, such as TaqMan or molecular beacons.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 200 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
Sigma Aldrich Fine Chemicals Biosciences fastStart TaqManR Probe Ma
Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore there is no elongation during the period when primers can nonspecifically bind. The fastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
Eagle Biosciences Inc E1A - SV40LTA Residual DNA Detection Kit (Multiplex qPCR)
E1A - SV40LTA Residual DNA Detection Kit (Multiplex qPCR)
BIOSEARCH TECHNOLOGIES INC (Lucigen) 2X BHQ Probe Master Mix, Standard ROX, 10mLA versatile mix for both end-point PCR genotyping and qPCR applications.
2X BHQ Probe Master Mix, Standard ROX, 10mLA versatile mix for both end-point PCR genotyping and qPCR applications.
New England Biolabs, Inc. WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) – 500 reactions (25 µl vol)
The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions. This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow. This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15-40 minutes.